G-418 DiSulphate Size:1g 2 at 25°C Suspend 28

2 at 25°C Suspend 28 gram powdered medium in 1 litre distilled water

Tryptone 10 Yeast extract 5 NaCl 0

8 gram powdered medium in 1 litre distilled water

Concentrations of Cysteine

4 Final pH 7

G-418 DiSulphate Size:1g 2 at 25°C Suspend 28C20H40N4O10. 2H2SO4=692. 7 G 418 is a member of the large family of Aminoglycoside antibiotics and has a molecular structure almost like Gentamycin. The antibiotic is an irreversible inhibitor of protein synthesis. Due to its structure is capable of binding to both prokaryotic and eukaryotic ribosomes and inhibiting protein synthesis and generating errors in the transcription of the genetic code. G 418 can by inactivated by NPTII or 3APH. The hydroxyl