Hygromycin B Powder Size:5g 3 g/L Store at room

3  g/L Store at room temperature

Hybridization between Escherich coli and Shigella

Tryptone 12 Yeast Extract 24 KH2PO4 2

LB-Broth High Salt

nucleotides and carbohydrates

Hygromycin B Powder Size:5g 3 g/L Store at roomEmpirical Formula (Hill Notation): C20H37N3O13 Molecular Weight = 527. 5 Hygromycin B is an aminoglycoside produced by Streptomyces hygroscopicus. The antibiotic interferes with the translation step of polypeptide synthesis of prokaryotes and eukaryotes. The antibiotic inhibits peptide chain elongation by preventing elongation factor EF 2 dependent translocation. Hygromycin B is used as a selective agent in yeasts, bacterial and plant cells. The