Yeast Vitamin Free Base Size:250g Used as a selective marker

Used as a selective marker for the transformation of plasmids encoding for tetracycline resistance (Tetr)

The medium is buffered by Disodium-β-glycerophosphate which prevents a drop in pH by the production of organic acids

This blocks the synthesis of deoxynucleotides

G-418 is a member of the large family of Aminoglycoside antibiotics and has a molecular structure almost like Gentamycin

LB-Broth Low Salt

Yeast Vitamin Free Base Size:250g Used as a selective markerYeast Nitrogen Base Vitamin Free is based upon the formulation of Yeast Nitrogen base except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins. Suspend 17. 0 gram powdered medium in 1 litre distilled water Store dry at room temperature