Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Pyridoxine Size:1000g Agar and Amino Acids

Agar and Amino Acids

coli and a higher yield of plasmid DNA compared to LB type media

2H2O = 372

Tryptone 10 NaCl 5 MgSO4 anhydrous 0

The antibiotic is inactivated by autoclaving and a solution should be sterilized by means of filtration

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Pyridoxine Size:1000g Agar and Amino AcidsPyridoxine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of pyridoxine or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room